Once you've unlocked all the businesses on a city's board, that city's Start tile transforms into a Travelling Merchant.
The Travelling Merchant sells business lootboxes (purchasable with bucks or cash) that drop Prestige Business NFTs — gold versions of that city's businesses that grant a permanent +20% revenue boost when owned.
A premium Gold Lootbox is also offered for real cash; it has better odds of dropping rarer prestige businesses than the standard lootboxes.